Composite
WT GFP

Part:BBa_K2918030:Design

Designed by: TUDelft2019   Group: iGEM19_TUDelft   (2019-10-04)


WT T7 promoter - Universal RBS - GFP - T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
    Illegal BamHI site found at 674
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MoClo Overhangs


Source

MoClo Assembly

References